Blasts with rosette-like multinucleation in acute myeloid leukemia with complex cytogenetic abnormalities.

A 28-year-old female presented with chills, sweats, and profound weakness. Laboratory findings revealed hypercalcemia, elevated lactate dehydrogenase, and pancytopenia. Bone marrow aspirate revealed 95% blasts with 2 distinct populations. A subset of blasts (20%) had typical morphology with smallto medium-sized nuclei, relatively regular nuclear outlines, and scant cytoplasm; however, the majority of the blasts were large and atypical with highly convoluted nuclear outlines, numerous cytoplasmic vacuoles, and binucleation/ multinucleation with rosette-like features. By flow cytometric analysis, both of the blast populations expressed CD4 (dim), CD13, CD15, CD33 (dim), CD36, CD64 (dim), and MPO (dim) without expression of HLA-DR, CD34, or CD117, findings indicative of acute myeloid leukemia with monocytic differentiation. There was no phenotypic evidence of megakaryocytic differentiation. Genetic studies revealed 5 clonal cell lines with complex cytogenetic abnormalities and FLT-3 D835 point mutation without FLT-3 internal tandem duplication, NPM1, or CEBPA.